Therapeutic Effect of Hydro-Ethanolic Extract of Pothos scandens L on key Carbohydrate Metabolizing Enzymes and Xenobiotic Marker Enzymes in DMBA Induced Experimental Mammary Carcinoma

Abstract:

Objective: In the present study, phytochemical evaluation and the therapeutic effect of 50 % hydro-ethanolic extract of aerial parts of Pothos scandens (HEEPS) on glycolytic enzymes, TCA cycle enzymes, gluconeogenic enzymes and xenobiotic marker enzymes in the liver and breast tissues of 7, 12 -Dimethylbenz [a] anthracene (DMBA) induced breast cancer bearing rats were carried out. Materials and Methods: Five groups of (each group six rats) female albino Spargue-Dawley rats were taken. Group I (control), Group II (DMBA induced, 20 mg/ kg b.wt p.o), Group III (DMBA + Cyclophoshamide, 10mg/kg b.wt p.o), Group IV (DMBA + 200 mg/kg b.wt p.o of HEEPS) and Group V (DMBA + 400 mg/kg b.wt p.o of HEEPS). The alteration in the level of carbohydrate metabolizing enzymes such as Hexokinase, Phosphogluco isomerase, Glucose-6-phosphatase, Fructose-1,6-bis phosphatase, Succinate dehydrogenase and Malate dehydrogenase together with the level of xenobiotic markers like Cytochrome P450 reductase, Cytochrome C oxidase and Glutathione -S- transferase were analyzed in all the groups. GC-MS analysis of the plant extract was also carried out. Results: Increased level of glycolytic enzymes, decreased level of TCA cycle enzymes, gluconeogenic enzymes and altered level of xenobiotic marker enzymes observed in Group II was brought to near normal in treatment groups in a dose dependent manner suggesting potential anticancer activity of HEEPS. This was supported by the GC-MS analysis of the plant extract which revealed the presence of many compounds with anticancer activity. Conclusion: The study revealed that HEEPS is effective in restoring the activity of mitochondria in DMBA induced breast cancer.

Key words: Pothos scandens L, GC-MS, 7, 12 -Dimethylbenz [a] anthracene (DMBA), Mammary Carcinoma, Carbohydrate Metabolizing Enzymes, Xenobiotic Markers.