Investigation of in vitro and in vivo Pursuits of Laurus nobilis Extract on Acetic Acid-induced Ulcerative Colitis in Wistar Rats

Indian Journal of Pharmaceutical Education and Research

  • Suliman Abdullah Al-Omran1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-ahsa, SAUDI ARABIA.
  • Virdala Chinnikrishnaiah2Department of Pharmacology, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Andhra Pradesh, INDIA.
  • Vakkalagadda Siva Ganesh3Department of Pharmaceutics and Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.G. Nagar, Karnataka, INDIA.
  • Prakash Goudanavar3Department of Pharmaceutics and Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.G. Nagar, Karnataka, INDIA.
  • Nimbagal Raghavendra Naveen3Department of Pharmaceutics and Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.G. Nagar, Karnataka, INDIA.
  • Santosh Fattepur4Department of Pharmacology, School of Pharmacy, Management and Science University, Selangor, MALAYSIA.
  • Girish Meravanige Basavarajappa5Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, SAUDI ARABIA.
  • Predeepkumar Narayanappa Shiroorkar5Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, SAUDI ARABIA.
  • Mallikarjun Telsang6Department of Surgery, College of Medicine, King Faisal University, Al-Ahsa, SAUDI ARABIA.
  • Sreeharsha Nagaraja1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-ahsa, SAUDI ARABIA., 7Department of Pharmaceutics, Vidya Siri College of Pharmacy, Off Sarjapura Road, Bangalore, Karnataka, INDIA.

Volume 57 Issue 3s Pages s644-s651

DOI: 10.5530/ijper.57.3s.73

Abstract

Background: The ethanolic extract of Laurus nobilis possesses an anti-inflammatory effect, which will protect against acetic acid-induced Ulcerative colitis by inhibiting endogenous free radicals. Objectives: The work has been undertaken to explore the efficacy and safety of ethanolic leaf extract of Laurus nobilis in treating acetic acid-induced Ulcerative colitis by estimating the biochemical, physical parameters, and histopathological studies. Materials and Methods: The plant powder of L. nobilis was extracted using 70% ethanol, and a phytochemical investigation was performed. Male and Female Wistar albino rats were divided into six groups and pretreated with saline, saline followed by ulcer induction, two doses of extracts (100mg/kg and 200mg/kg b.w), and sulfasalazine (one day) followed by ulcer induction and one low dose extract treatment without inducing ulcer. Results: The phytochemical investigation of the extract confirms the presence of flavonoids. The biochemical estimations showed increased SOD, CAT, and GSH and decreased levels of TBARS in extract-treated groups compared to the acetic acid-induced group. The physical parameters like Crypt abscesses, gross mucosal inflammation, wet weight of the colon, diarrhea, and activity score have also shown better results in extract-treated groups showing a protective effect. Histopathological results further confirmed the therapeutic effect of the extract. Conclusion: The protective effect of ethanolic leaf extract of Laurus nobilis has been proved. The therapeutic effect was mainly due to flavonoids, and the low-dose extract was more potent than the high-dose extract.

Keywords

  • Laurus nobilis
  • Ulcerative Colitis
  • Anti-oxidant
  • Histological Studies
  • Ethanolic Extract
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