Development and Validation of UV-spectrophotometric Method for the Estimation of Wintergreen Oil in Pharmaceutical Formulation

Indian Journal of Pharmaceutical Education and Research

  • Deepali1 Department of Pharmaceutics, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.
  • Vijaya G Joshi1 Department of Pharmaceutics, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.
  • S Ramachandra Setty2 Department of Pharmacology, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.
  • M Geetha1 Department of Pharmaceutics, Government College of Pharmacy, Bengaluru, Karnataka, INDIA.

Volume 57 Issue 2 Pages 591-597

DOI: 10.5530/ijper.57.2.72

Abstract

Objectives: The study aimed to develop and validate a simple UV-spectrophotometric method for quantifying Wintergreen oil in micro/nanosponges. Materials and Methods: The method was developed using acetonitrile as a solvent system and validated for various parameters such as linearity, precision, repeatability, the Limit of Detection (LOD), and the Limit of Quantification (LOQ), and accuracy according to ICH guidelines. Results: The oil showed an absorption maximum at 237nm. Linearity between concentration and absorbance was established within a concentration range of 2 to 28 mcg/mL and showed a regression coefficient of 0.9746. The recovery of 97.14-107.82% and % RSD of less than 2% for repeatability, intraday, inter-day, and ruggedness revealed that the method is accurate and precise. The LOD and LOQ were determined as 0.103 mcg/mL and 0.312 mcg/mL respectively. Insignificant changes in absorption values with deliberate variation in absorption maximum indicate the method's robustness. The entrapment efficiency and loading capacity ranged between 88-97.82% and 3.085-24.35%. Conclusion: The validation results conclude that the method is simple, sensitive, precise, accurate, and robust. Hence, the method was used for quantifying wintergreen oil in micro/nanosponges. The procedure can further be adapted for analysing wintergreen oil in other pharmaceutical preparations and commercial products.

Keywords

  • Wintergreen oil
  • UV-spectrophotometry
  • Validation
  • Micro/nanosponges
  • Pharmaceutical formulation
IJOPP

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