Morus rubra Extract Induces G1 Cell Cycle Arrest and Apoptosis in Human Lung and Prostate Cancer Cells

Indian Journal of Pharmaceutical Education and Research

  • Ibrahim Turan1Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University, 29100, Gumushane., 2Medicinal Plants, Traditional Medicine Practice and Research Center, Gumushane University, 29100, Gumushane.
  • Selim Demir3Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080, Trabzon.
  • Kagan Kilinc1Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University, 29100, Gumushane.
  • Yuksel Aliyazicioglu4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon.
  • Ahmet Alver4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon.
  • Sema Misir4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon., 5Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University, 58140, Sivas.
  • Serap Ozer Yaman4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon.
  • Kubra Akbulut4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon.
  • Ahmet Mentese6Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Orhan Deger4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon.

Volume 51 Issue 1 Pages 51-58

DOI: 10.5530/ijper.51.1.8

Abstract

Objective: Cancer is one of the most deadly types of disease and evasion from apoptosis and unstoppable cell proliferation are accepted as its distinctive features. Many studies have evaluated the cytotoxic effect of different Morus species but, there is no study about cytotoxic effect of Morus rubra. In this study we aimed to evaluate phenolic composition, antioxidant properties and cytotoxic effect of acidified dimethyl sulfoxide extract of M. rubra (AMRE). Method: Antioxidant properties, phenolic composition and cytotoxic effect of AMRE were determined using spectrophotometric methods, HPLC, and MTT assay, respectively. Then, mechanisms of cytotoxic effect of AMRE on human prostate (PC-3) and lung (A549) cancer cells were examined in regard to cell cycle, apoptosis and mitochondrial membrane potential using flow cytometric methods. Results: Total phenolic content and reducing power values were 11.9 mg gallic acid equivalents and 42.9 mg trolox equivalents per g sample, respectively. Ascorbic and gallic acid were detected in AMRE as major antioxidant compounds. We determined that AMRE increased cell cycle arrest at G1 phase and exhibited apoptotic features via decreasing mitochondrial membrane potential in both prostate and lung cancer cells. Conclusion: These findings demonstrate that M. rubra extract can affect the behavior of human prostate and lung cancer cells in vitro conditions, and this effect now needs to be investigated in vivo.

Keywords

  • Apoptosis
  • Cell Cycle
  • Cytotoxicity
  • Lung Neoplasms
  • Morus rubra
  • Prostate
  • Neoplasms
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