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Published on:August 2016
Indian Journal of Pharmaceutical Education and Research, 2016; 50(3):397-402
Original Article | doi:10.5530/ijper.50.3.12

Chondroprotective and Anti-Inflammatory Activities of Extracts from Semen Sojae Germinatum on IL-1β- Stimulated Human Osteoarthritis Chondrocytes

Authors and affiliation (s):

Wenhui Fan1, Ying Qian1, Jun Wang1, Xiliang Yang1, Tong Gui2* and Bingshu He2

1Department of Pharmacy, College of Medicine, Wuhan University of Science and Technology, Wuhan-430065, CHINA.

2Department of Orthopedic Surgery, Hubei Woman and Child Hospital, Wuhan-430070, CHINA.


Our previous study showed that Semen sojae germinatum (SSG), a soy-derived Chinese medicinal material, have potential benefits for knee osteoarthritis (OA). This study was undertaken to identify the major effective sub-fraction of SSG. The 95% ethanol extract of SSG was successively fractionated into petroleum ether, ethyl acetate, n-butanol and aqueous fractions. Then we examined the effect of fractions on the inflammatory response and cell proliferation of primary human osteoarthritic chondrocytes stimulated by interleukin (IL)-1β, a recognized and common inducer of OA in vitro. Results showed that the petroleum ether, ethyl acetate and n-butanol extracts of SSG promoted cell-proliferation measured by MTT [3-(4,5- dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide] assay, increased the transcript levels and nuclear translocation of cyclin D1, and inhibited the production of pro inflammatory mediator prostaglandin (PG)E2 and nitric oxide (NO) induced by IL-1β in human OA chondrocytes, suggesting SSG extracts possessed chondro protective and anti-inflammatory properties. The ethyl acetate fraction was superior to other fractions. These data indicated SSG extracts might become a potential treatment option for humans with OA.

Key words: Semen sojae germinatum, Extract, Osteoarthritis, Chondrocytes, Inflammatory response, Proliferation.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
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Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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