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Published on:May 2023
Indian Journal of Pharmaceutical Education and Research, 2023; 57(2s):s321-s326.
Original Article | doi:10.5530/ijper.57.2s.37

Role of Remifentanil on MAC, Bcl-2, and Bax Levels in Traumatic Brain Injury Rat Models


Authors and affiliation (s):

Ardana Tri Arianto1,*, Soetrisno2, Purwoko1, Dono Indarto3

1Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, INDONESIA.

2Doctorate Program of Medical Sciences & Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, INDONESIA.

3Doctorate Program of Medical Sciences and Department of Physiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, INDONESIA.

Abstract:

Introduction: Traumatic Brain Injury (TBI) conditions require immediate treatment such as the administration of analgesics and sedation, including remifentanil. TBI will affect the levels of several inflammatory and apoptotic proteins such as MAC, Bax, and Bcl-2. Remifentanil with its high affinity to μ-receptors is thought to affect the levels of these proteins and has neuroprotective properties to prevent secondary brain injury. Objectives: This research aims to determine the effect of giving remifentanil on MAC, Bcl-2, and Bax levels in rats with traumatic brain injury. Materials and Methods: Experimental research was conducted on 24 male Wistar rats, which were randomly divided into four groups. The Group-1 (G1) received an intravenous injection of NaCl, the Group-2 (G2) was given normal saline after TBI induction using the modified Feeney method. Furthermore, the Group-3 and Group-4 (G3 and G4) received fentanyl or remifentanil after TBI induction. The rat brain tissue was analyzed for MAC, Bcl-2, and Bax levels using the ELISA method. The data were analyzed statistically by ANOVA test, followed by Post-Hoc Multiple Comparison Test (p<0.05). Results: The MAC and Bax levels were reduced by the administration of fentanyl and remifentanil to TBI subjects, but increased Bcl-2 levels. The Post Hoc Multiple comparisons showed a significant difference between all group pairs (p<0.05). Meanwhile, the group given remifentanil showed more optimal results than those with fentanyl. Conclusion: The administration of remifentanil injection can act as a neuroprotector in TBI model Wistar rats by decreasing MAC and Bax levels and increasing Bcl-2 levels.

Keywords: Bax, Bcl-2, MAC, Remifentanil, Traumatic brain injury.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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