Production of L-tyrosinase from Novel Variants of Streptomyces cellulosae

Abstract:

Background: Recently, a lot of interest has been focused on the discovery of high L-tyrosinaseyielding microorganisms that catalyze the production of enantiomerically pure L-DOPA, which has been recognized as the conventional treatment for Parkinson's disease. Objectives: The current work primarily focused on isolating novel, promising tyrosinase-producing isolates and performing taxonomical characterization on them. Materials and Methods: Initially six various actinomycetes isolates were used in present study. Primary screening and secondary screening of selected isolates were performed. By evaluating the morphological, cultural, physiological, biochemical and molecular characterization, promising isolates were identified. Using 16S rRNA gene sequencing, the isolates' Phylogenetic investigations were conducted. Results: Streptomyces cellulosae variants, isolates PD18 and PD26 were identified to be the potential actinomycete isolates and their respective maximal tyrosinase yields were reported to be 21.20 IU/mL and 24.19 IU/mL. Conclusion: According to the observations of the experiment, SS (Soluble starch) medium with 1mg/mL copper sulphate was most suitable medium for tyrosinase production from our promising isolates PD18 and PD26. The maximum yield was noticed after 168 hr of incubation. This work emphasizes the significance of finding novel variants of Streptomyces cellulosae, which were first used to produce tyrosinase as well as promising sources for potentially bioactive substances like antimicrobial metabolites, biosurfactants and enzymes like amylase, lipase, cellulase, uricase, urease, β- galactosidase and L- asparaginase.

Keywords: Tyrosinase, L- tyrosine, L-DOPA, SS medium, copper sulphate, Streptomyces cellulosae.