Evaluation of Methanolic Extract of Polyherbal Cream on DNCB-induced Atopic Dermatitis in Wistar Albino Rats

Abstract:

Background: Atopic dermatitis, is a multifactorial chronic inflammatory and immunological disease affecting the skin, with the serious impact on psychological and life style of the patients. Unique polyherbal combination of Aloe vera, Erngium foetidum, Passiflora edulis, Syzygium samarangense, based on established profile of relevant to anti-inflammatory, immunomodulatory and anti-oxidant property and formulated as cream for effective therapy. Materials and Methods: Wistar albino rats were induced AD by sensitisation of DNCB-BSA on 1^st day of 1^st week followed by re-sensitisation of 1^st day of each week for additional 5 weeks to all group except control with intervention of Pimecrolimus and low and high dose of MEPC for group-3, 4 and 5 respectively, however for group-1 and 2 no treatment was given. Parameters such as scratching behaviour, frequency and ear thickness are recorded on a weekly basis, whereas the Serum total and IgE, ear weight and histopathology were done at the end (42^nd day) of experiment. Results: MPEC has significant and dose dependent decrease in all parameters, the standard drug has highest positive results with nearly equivalent result of high dose of MEPC in parameters such as scratching behaviour, frequency, IgE and histopathology, however as in the case of ear weight high dose of MEPC has more positive results than Pimecrolimus (standard drug), reflecting MEPC has more immunomodulatory effect in long term use as compare to Pimecrolimus. Conclusion: MEPC might provide good alternative therapy against the treatment of AD to improve the life style of patient without ADR.

Keywords: Atopic Dermatits, Methanolic Extract of Polyherbal Cream, Gas Chromatography-Mass Spectrometry, 2,4-dinitrochlorobenzene, Dinitrophenyl-bovine serum albumin, Acetone-olive oil solution.