Formulation and Characterization of Chrysin Loaded Phytosomes and its Cytotoxic Effect against Colorectal Cancer Cells

Abstract:

Background: Chrysin is a phytoconstituent which has anticancer activity. The study aims to formulate, characterize and evaluate the cytotoxic effect of chrysin loaded phytosomes against HT29 cells. Materials and Methods: Antisolvent precipitation technique was employed to prepare phytosomes. Particle size, polydispersity index, zeta potential, entrapment efficiency, scanning electron microscope and Fourier transform infrared spectroscopic analysis were carried out for the characterization of chrysin loaded phytosomes. Cell viability was done to evaluate the cytotoxic effect of developed phytosomes comparing with plain chrysin. Results: The developed chrysin loaded phytosomes showed the particle size of 94.40nm, polydispersity index of 0.31, and zeta potential -1.33 mV. The entrapment efficiency was 74.28 %. Chrysin loaded phytosomes showed increased cytotoxic effect on HT-29 cells. Conclusion: This research work produces confirmative indication for the use of Chrysin loaded phytosomes in experimental animals to further gain in depth analysis for anticancer activity of chrysin loaded phytosomes against colon cancer.

Keywords: Chrysin, Phytoconstituent, Nanoformulation, Colorectal cancer, Cytotoxicity.