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Published on:July 2022
Indian Journal of Pharmaceutical Education and Research, 2022; 56(3s):xx-xx
Original Article | doi:

Investigation of Memory Enhancing Activity of Madhuca longifolia Leaf Extract against Colchicine: An Experimental Study and Biochemical Alterations in Mice

Authors and affiliation (s):

Pragati Khare1,*, Kamal Kishore2, Dinesh Kumar Sharma3

1Department of Pharmacy, Shri Ram Murti Smarak, C.E.T., Bareilly, Uttar Pradesh, INDIA.

2Department of Pharmacy, M.J.P. Rohilkhand University, Bareilly, Uttar Pradesh, INDIA.

3Department of Pharmacy, Himalayan Institute of Pharmacy and Research, Dehradun, Uttarakhand, INDIA.


Objectives: The present study aimed at investigating the protective role of Madhuca longifolia ethanolic leaf extract flavonoid fraction against colchicine induced cognitive dysfunction and oxidative damage in swiss albino mice and to estimate the biochemical alterations in mice brain. HPTLC, total flavonoid and total phenols were also estimated in the study. Materials and Methods: The analysis was conducted on a colchicine-induced model for 28 days. Morris water maze and passive avoidance paradigm were utilized for behavioral experiments, while biochemical parameters such as nitric oxide and glutathione were estimated. Swiss albino mice (48) were apportioned into eight sets, each consisting of six mice. 1 percent w/v carboxy methyl cellulose was apportioned to the first Group. The second group got 200 milligrammes/kg of Piracetam i.p. Group III received 1 mg / kg i.c.v. colchicine. The fourth and fifth set of groups was given 100 and 200 mg / kg ethanolic Madhuca longifolia leaf extract. Group VI has been given Piracetam (200 mg / kg, i.p.) for 28 days and Colchicine 1 mg / kg, i.p., at 60 min after 28th day piracetam injection. Group VII and VIII obtained oral extracts of Madhuca longifolia leaf 100 and 200 mg/kg for 28 days and injected colchicine (1 mg / kg) i.c.v for 90 mins after extract administration on the 28th day. ANOVA (one-way) was utilized and then followed by the test of Dunnett’s and finally outcomes analyzed. Results: Madhuca longifolia leaf extract indicated morris water maze and had a substantial reduction in transfer latency. The transfer latency of the passive avoidance model showed a substantial increase. The Madhuca longifolia leaf extract presented a substantial increase (P<0.001) in GSH intensities and a major decline (P<0.001) in total protein and NO. Conclusion: Madhuca longifolia has neuroprotective effect against memory damage caused by colchicine.

Key words: Madhuca longifolia, Neuroprotective, Alzheimer’s disease, Colchicine, HPTLC, Cognition.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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