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Published on:July 2022
Indian Journal of Pharmaceutical Education and Research, 2022; 57(4s):xx-xx
Original Article | doi:

Development Optimization and Cytotoxicity Evaluation of Glyburide Loaded Nanostructured Lipid Carriers


Authors and affiliation (s):

Ashwini M1,*, Preethi Sudheer1, Bharani S. Sogali2, Chandramouli R3

1Department of Pharmaceutics, Krupanidhi College of Pharmacy, Bengaluru, Karnataka, INDIA.

2City Health Care Hospital, Kolar, Karnataka, INDIA.

3Department of Quality Assurance, Krupanidhi College of Pharmacy, Bengaluru, Karnataka, INDIA.

Abstract:

Aim/Background: In the present study Glyburide, a hypoglycaemic agent was loaded into nanostructured lipid carriers. The solid and liquid lipid concentrations within the formulation were optimized using central composite design with two centre points augmented with a cluster of axial and factorial points (star points). Materials and Methods:The responses particle size and drug entrapment were used to optimize the concentration ratio by central composite design. The nanostructured lipid carriers were prepared by melt dispersion technique and the optimized formulations were evaluated for various physicochemical, morphological, histological and toxicity parameters. Results: Central composite design paradigm was successful in defining the best lipid concentrations. Scanning electron microscopy and atomic force microscopy revealed the morphological characteristics of the nanoparticle. The outcome of X- ray diffraction confirmed the molecular dispersibility of the drug in the lipid matrix. Histopathological study conceded the scope of formulating a transdermal delivery system using the optimized nano carrier. Cell viability study in baby hamster kidney-21 cell culture substantiated the non-toxicity of the nanoparticles. Conclusion: Study construed the suitability of the design model in optimization and assessment of the impact of the concentration of solid lipid as well as liquid lipid on the responses particle size and drug entrapment efficiency.

Key words: Glyburide, Central composite design, MTT assay,Cell viability study, BHK -21 cell-line, Histopathological study.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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