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Published on:March 2022
Indian Journal of Pharmaceutical Education and Research, 2022; 56(2):497-502
Original Article | doi:10.5530/ijper.56.2.71

Bioguided Isolation of Alternariol Derivatives from Ficus-derived Endophyte Alternaria alternata


Authors and affiliation (s):

Randa Abdou1,2,* Gouda H Attia3, Mariam Mojally4, Mohamed Dawoud5, Mostafa E Rateb6

1Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, Makkah, SAUDI ARABIA.

2Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, EGYPT.

3Department of Pharmacognosy, Faculty of Pharmacy, Najran University, Najran, SAUDI ARABIA.

4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, SAUDI ARABIA.

5Department of Pharmaceutics, Faculty of Pharmacy, Umm Al-Qura University, Makkah, SAUDI ARABIA.

6School of Computing, Engineering and Physical Sciences, University of the West of Scotland, Paisley PA1 2BE, U.K.

Abstract:

Background: Endophytes are a rich source of bioactive natural products and suggested to contribute to the biological or defense activities of their host plants. Following our research on the discovery of bioactive metabolites from endophytes, Alternaria alternata was isolated from the leaves of Ficus carica L. fam Moraceae. Materials and Methods: Large scale cultivation of the endophytic strain was carried out and the obtained extract was subjected to preliminary screening of antifungal and cytotoxic activities. Results: Promising antifungal and cytotoxic activities were obtained for the extract. Bio-guided fractionation resulted in the isolation and identification of four alternariol derivatives (alternariol, alternariol-5-O-sulphate, alternariol-5-O-methyl ether, alternariol-5-O-methyl ether-4`-O-sulphate). The isolated compounds were tested for antifungal and cytotoxic effects. Results revealed highest antifungal activity for alternariol against A. terreus (MIC= 2.64 μg mL-1) and F. oxysporum (MIC= 36 μg mL-1) while alternariol-5-O-methyl ether exhibited the highest cytotoxicity against K-562 (CC50= 3.72 μg mL-1) and HUVEC (CC50= 2.06 μg mL-1) cell lines. Conclusion: All alternariol derivatives showed potent cytotoxic and antifungal activities against A. terreus suggesting the contribution of this endophyte in the known antimicrobial and anticancer activities of the host plant.

Key words: Endophyte, Alternariol, Alternaria alternata, Anticancer, Ficus carica.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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