Antimicrobial, Antioxidant and Cytotoxic Activities of Different Fractions Obtained from Endophytic Fusarium solani F01 Isolated in Catharanthus roseus Collected in Vietnam

Abstract:

Aim: Finding out the biological sources is essential for pharmaceutical field. Materials and Methods: Endophytic fungus was isolated from Catharanthus roseus originated in Vietnam and then identified by comparing, phylogeny analyzing its 18S sRNA sequence using Blast search and GenomeNet. The supernatant and sonicated mycelia were fractionated with methanol, ethyl acetate, hexane. All the fractions were used to test antimicrobial, antioxidant, cytotoxicity activities. Antimicrobial activities on Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Serratia marcescens ATCC 14756, Vibrio parahaemolyticus ATCC 17802, and Escherichia coli ATCC 25922 were performed by well diffusion method. In terms of antioxidant activities, reducing power and DPPH radical scavenging assay were done. The purification from methanol extract was performed. The purified compounds were used to test antioxidant activity. For cytotoxicity activity, sulforhodamine B assay was used to test the extracts and purified compounds on the breast cancer cell line (MCF-7). Results: Fusarium solani F01 was identified. The treated methanol extract from cell-free supernatant showed the highest activities and wider spectrum than ethyl acetate and hexane extracts. Mycelial extracts in these solvents did not show activities except ethyl acetate extract. The methanol crude extract from cell-free supernatant gave the higher reducing power (1.416 ± 0.067) than ascorbic acid (0.577 ± 0.01) while others were very low at the concentration (100 μg/ ml). Only methanol extract obtained from supernatant showed moderate scavenging activity when comparison of their IC₅₀ (9.17 g/ml) with IC₅₀ of ascorbic acid (1.28 g/ ml). Four compounds were obtained that showed antioxidant activities, but there was one purified compound showed low effects on MCF-7 while methanol extract gave the highest inhibition (80.34 ± 1.28). Conclusion: The study suggested fungus isolated from Catharanthus roseus could show and give many biological compounds that will be a potential source for pharmaceutical field.

Key words: Catharanthus roseus, Identification, Molecular analysis, Biological activities, Extraction, Purification.