Home | Articles
Published on:October 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(4S):xx-xx
Original Article | doi:10.5530/ijper.55.xx

Optimization of a Validated UV-Spectrophotometric Methodology for Assessment of Apigenin in Bulk Powder


Authors and affiliation (s):

Priya Shetti1, Sunil S. Jalalpure2,*

1Dr. Prabhakar Kore Basic Science Research Center, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi, Karnataka, INDIA.

2Department of Pharmacognosy, KLE College of Pharmacy Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi, Karnataka, INDIA.

Abstract:

Introduction: Apigenin (4,5,7 Trihydroxyflavone) is a natural compound of flavonoids class, which is found in various plant constituents. Since primitive years, Apigenin has been used as a traditional medicine, because it possesses biological functions. Common ayurvedic formulations comprising apigenin are in market. Hence quality control of preparation covering apigenin is desirable. Objectives: In present study, pointed to enhance and validate UV Spectrophotometric method to evaluation of apigenin in bulk powder. Methods: Using methanol as solvent, UV Spectrophotometric method was developed. Apigenin showed supreme absorbance wavelength at 267 nm. New technique enhanced and validated in terms parameters such Specificity, Discrimination, Linearity, Correctness, Ruggedness, Solution stability as per each ICH guidelines. Results: The detector response for apigenin was linear in the sure concentration range of 2μg/mL-10μg/mL with correlation coefficient of 0.9995. Newly developed method was found to be specific, Selective linear, Precise, Rugged, reproducible for estimation of apigenin with %RSD values less than 2%. Conclusion: In this study Development and Validation of UV-Spectrophotometric method can be employed for the apigenin assessment in bulk powder.

Key words: Apigenin, Absorbance, ICH guidelines, Ruggedness, Spectrophotometric, Ayurvedic Formulations.

 




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the Latest Release of Journal Citation Reports (source: Web of Science Data).

 

Impact Factor® as reported in the 2020 Journal Citation Reports® (Clarivate Analytics, 2021): 0.638

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs