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Published on:September 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(3s):xx-xx
Original Article | doi:10.5530/ijper.54.3s.x

Anti-metastatic and Anticancer Potentials of Synthesized Chalcones in B16-F10 Melanoma Cells Induced Metastatic Lung Cancer in C57BL/6 Mice


Authors and affiliation (s):

Nitesh Kumar1,2$, Nunsavath Kishan1$, Subhankar Biswas1, Karthik Gourishetti1, Mehnaz Kamal3, Mallikarjuna Rao Chamallamudi1,*

1Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, INDIA.

2Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Hajipur, Bihar, INDIA.

3Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj -11942, KINGDOM OF SAUDI ARABIA.

Abstract:

Aim/Background: Metastasis is the main reason of death in cancer with a key challenge to target it precisely by the available treatments. The current study was designed to assess the anti-metastatic potentials of the synthesized chalcones, Ch-1 and Ch-2. Materials and Methods: In vitro study included cytotoxicity, scratch wound assay and cell cycle analysis of Ch-1 and Ch-2. In vivo study was performed in C57BL/6 mice in a B16-F10 induced metastatic lung cancer model. Results: Ch-1 was found to be a potent cytotoxic agent on cell lines namely, MCF-7 (human breast cancer cells), B16-F10 (melanoma cells), Hela (cervical cancer cells) and HEK (human embryonic kidney cells) with IC50 < 100 μM. Ch-1 deterred the G2/M phase of the MCF-7 cell cycle, S Phase of the HeLa cell cycle and G0-G1 phase of the B16-F10 cell cycle. Scratch wound assay in MCF-7 cells with Ch-1 and Ch-2 showed a significant (p<0.05) prevention in their multiplication when compared to untreated cells at both 24 h and 48 h intervals. In vivo study showed that, oral Ch-1 administration at 100 mg/kg b.w. reduced metastatic lung nodules to 77.32%. Standard drug Quercetin at 100 mg/kg b.w. inhibited the lung tumor nodule formation to 56.5%. However, except for WBC count, Ch-1 (100 mg/kg) also displayed significant improvement in hematological profile. No significant difference was found in any of the groups of animals in mean body weight and major organ weight. Conclusion: Study supports the possible use of Ch-1 as a potential anticancer agent in metastatic lung cancer.

Key words: Chalcones, Anticancer, B16-F10 melanoma cells, Metastatic lung cancer, Cell Cycle.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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