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Published on:1 January, 2012
Indian Journal of Pharmaceutical Education and Research, 2012; 46(1):70-74
Research Article | doi:Nill

Studies on Production of 6-Aminopenicillanic Acid by Free and k- Carrageenan Immobilized Soil Bacteria


Authors and affiliation (s):

Ashoke Kumar Dolui*, (Late) Sitikantha Sahana and Atul Kumar

Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, 786004, Assam, India

Abstract:

In the present study different bacterial samples from the soil of different places of Dibrugarh were isolated and incubated in presence of penicillin G (2 mg/ml) for 48 hours at 37°C for biotransformation to 6-amino penicillanic acid which was detected by Thin Layer Chromatography (TLC). Among 15 isolated bacterial samples, two gram positive bacterial samples designated as AKDD-7 and AKDD-9 produced 6-APA from penicillin G. Assessment of production of 6-APA was carried out by two selected samples separately in free and k-Carragennan immobilized state through HPLC in first stage after incubation in penicillin G (2 mg/ml) for 48 hours. Reusability of immobilized cells was found successful for production of 6-APA from penicillin G after one-day cold-preservation and another seven days' cold preservation in the second and third stage respectively.

Keywords: Penicillin G, 6- Aminopenicillanic acid, Cell immobilization, Bioconversion, HPLC assessment, k-Carragennan.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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