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Published on:March 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(1s):s285-s292
Original Article | doi:10.5530/ijper.55.1s.61

Development and Validation of UV Spectrophotometric Method for Estimation of Tea Tree Oil in Bulk and Cosmeceutical Creams


Authors and affiliation (s):

Basappa Palur Manjula, Vijaya Gopalachar Joshi, Ramachandra Setty Siddam Setty, Muniyappa Geetha

Department of Pharmaceutics, Government College of Pharmacy, Rajiv Gandhi University of Health Sciences, Bengaluru, Karnataka, INDIA.

Abstract:

Background: Tea tree oil, also known as Melaleuca alternifolia essential oil is widely used in skin care cosmeceuticals for its antibacterial, antifungal, analgesic and anti-inflammatory effect. The complex and variable composition of the oil poses many challenges to the analytical chemist and a recent survey of analytical methods indicated only a few simple and validated methods for estimation of the oil. Aim: A quality by design approach has therefore been adopted to develop a simple and novel UV spectrophotometric method for estimation of tea tree oil in bulk and cosmeceutical creams. Materials and Methods: UV spectrophotometric method was developed for estimation of Tea tree oil using dichloromethane- methanol (54 % v/v) solvent system. The method was then validated under optimized conditions for accuracy, precision and ruggedness, limit of detection and limit of quantification as per ICH: Q2 (R1) guidelines. Results: A characteristic spectral peak was observed at 267 nm and linearity was established over a concentration range of 10-160 mcg/ml with R2 value of 0.9961. Accuracy based on recovery studies, 100.5-113.6 %, precision and ruggedness based on % RSD values less than 2 %, LOD, 0.1277mcg/ml and LOQ and 4.195 mcg/ml indicated that the method is sensitive enough to be used for routine estimation. Conclusion: The method is robust and has been used to determine the essential oil content of two cosmeceutical creams.

Key words: Tea tree oil, UV spectrophotometry, Box Behnken design, Optimization, Routine determination, Cosmeceutical cream.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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