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Published on:February 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(1):215-223
Original Article | doi:10.5530/ijper.55.1.24

Ameliorative Effect of Multi Herbal Formulation on Lipid Peroxidation and Redox Dysfunction in Ethanol Induced Hepatic Imbalance


Authors and affiliation (s):

Soumendra Darbar1, Srimoyee Saha1, Kaushikisankar Pramanik2, Atiskumar Chattopadhyay3,*

1Faculty of Science, Jadavpur University, Raja SC Mallick Road, Kolkata, West Bengal, INDIA.

2Department of Chemistry, Jadavpur University, Raja SC Mallick Road, Kolkata, West Bengal, INDIA.

3Principal Secretary Faculty of Science, Jadavpur University, Raja SC Mallick Road, Kolkata, West Bengal, INDIA.

Abstract:

Introduction: Urolithiasis has afflicted human race since the days of yore. Conventional treatment acts only on associated ailments whereas pharmacologically active phytoconstituents derived from plants are reported to possess lithotriptic as well as anti-urolithiatic effect, thus herbal sources need to be scientifically explored. Present study was aimed to evaluate anti-urolithiatic potential of leaves of Alstonia scholaris and its isolated pentacyclic triterpenoids: lupeol and ursolic acid in ethylene glycolinduced renal calculi rat model. Materials and Methods: Leaves of Alstonia scholaris were extracted with various solvents, amount of triterpenoids in each extract was quantified using HPTLC. Extract which had higher amount of triterpenoid was evaluated for its anti-urolithiatic effect. Albino wistar rats of either sex were divided into 6 groups. All groups except vehicle were fed with 0.75%v/v of ethylene glycol for 28 days. Treatment with standard cystone, low and high dose of extract and pentacyclic triterpenoids were given to respective groups from 4th-28th day of study. On 28th day, serum creatinine, uric acid, BUN, TNF-α, IL-6 and urinary electrolyte levels were estimated. Kidneys were isolated, one was used for histopathological study and other for antioxidant assays. Results and Discussion: Histopathological examination of kidneys revealed destructed morphology due to deposition of large polymorphic crystals and cystic dilatation of tubules. Whereas treatment with extract and its pentacyclic triterpenoids prevented renal damage and deposition of crystals. Perturbations in levels of nitrogenous wastes, inflammatory mediators and urinary electrolytes were also prevented. Alstonia scholaris rich in triterpenoid significantly arrested associated oxidative stress and inflammation thus preventing formation of renal calculi. Conclusion: Leaves of Alstonia scholaris and pentacyclic triterpenoids isolated from them demonstrates potent herbal remedy for treatment of urolithiasis.

Key words: Alstonia scholaris, Anti-inflammatory, Lupeol, Oxidative stress, Ursolic acid, Urolithiasis.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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