Structural Elucidation of Isolated Phytochemicals from Selected Medicinal Plants with Antihyperlipidemic Activity and Development of Polyherbal Formulation with Densitometric Analysis of Isolated Phytoconstituents

Abstract:

Objectives: The present systematic research aimed to express the antihyperlipidemic effects of different types of herbs based on animal studies. The purpose of the present project is to perform the phytochemical investigation and exploration of hypolipidemic activity with the development and quantification of phytoconstituents of polyherbal hypolipidemic formulation employing Apium graveolens, Salvadora persica, Carica papaya and Evolvulus alsinoides. Materials and Methods: Flash chromatography technique was used for the isolation of phytoconstituents from the selected medicinal plants. Antihyperlipidemic study was done on triton induced model. Triton- WR 1339 (400 mg/kg b.w) i.p. injection were given to different animal groups. HPTLC densitometric method was established for the quantification of the phytoconstituents in polyherbal formulation. Results: The phytoconstituents so isolated were identified as 8, 8-Dimethyl-2H, 8H-pyrano [2, 3-f] chromen-2-one, 9- methoxyfuro [3, 2-g] chromen- 7-one, 7- hydroxy-2H-chromen -2-one, Isothiocyanatomethylbenzene and 17-(5-Ethyl- 6-methylheptan-2-yl)-10,13-dimethyl2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro 1Hcyclopenta[α]phenanthren-3-ol through chromatographic separation techniques and their structures were elucidated by spectroscopic analysis. The formulation showed significant reduction in TC, TG, LDL and VLDL level (p<0.01) and significantly raised HDL level (p<0.01) compared to hyperlipidemic group. Furthermore, HPTLC method was established for the quantification of isolated phytoconstituents in polyherbal formulation through densitometric analysis. Conclusion: The present work also characterized active phytoconstituents from different parts of selected plants. The research work involved development of polyherbal hypolipidemic formulation with a view to reduce the serum levels of TC, TG, LDL and to increase the serum level of HDL cholesterol and to establish HPTLC densitometric method for quantification of its phytoconstituents.

Key words: Hyperlipidemia, Medicinal Plants, Flash Chromatography, Isolated Phytoconstituents, HPTLC Densitometric Analysis.