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Published on:May 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(2s):s295-s300
Original Article | doi:10.5530/ijper.54.2s.86

In-vitro Cytotoxicity and in silico Molecular Docking of Alkaloids from Tiliacora acuminata


Authors and affiliation (s):

Jeswiny Rodrigues1, Kirankumar Hullatti1,*, Sunil Jalalpure1, Pukar Khanal2

1Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.

2Department of Pharmacology and Toxicology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.

Abstract:

Background: The present study aimed to identify the cytotoxicity of Tiliacora acuminata extract/fraction(s) plant via bioactivity guided approach and predict the binding affinity with Topoisomerase II. Materials and Methods: Extract and fractions were screened using brine shrimp lethality bioassay and the potent fraction was further evaluated for its in vitro cytotoxicity using five different cell lines i.e. HT-29, HepG2, MCF-7and A-549. The binding affinity of individual phytoconstituent from the potent fraction with Topoisomerase II was further predicted using autodock4. Results: The fraction containing alkaloids showed the highest cytotoxicity against the HT-29 cell line. Molecular docking study identified coclaurine as a potent alkaloid phytoconstituent to bind with topoisomerase II. Conclusion: The study revealed the potential cytotoxic alkaloids from T. acuminata which may find use in the development of cytotoxic or chemopreventive agents.

Key words: Alkaloids, Brine shrimp, Docking, Menispermaceae, MTT, Topoisomerase II.

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