In-vitro Anthelmintic Impact of Various Extracts of Pavetta tomentosa Root on Pheretima posthuma and in-silico Molecular Docking Evaluation of some Isolated Phytoconstituents

Abstract:

Background: The current study assesses the anthelmintic impact of root extracts of Pavetta tomentosa on Pheretima posthuma compiled by molecular docking analysis of phytocompounds steemed from the plant with the β-Tubulin (PDB ID: 1SA0). Methods: In this study, P. tomentosa root was subjected to extraction using methanol and water. In vitro, anthelmintic activity was assessed by utilizing the Pheretima posthuma and in silico molecular docking was executed making use of Autodock 4.0. Results: The outcomes revealed that the methanolic extract has the most significant dose-dependent anthelmintic activity at various doses, followed by aqueous extracts of root. Amongst all the substances, β-eudesmol revealed the most effective docking rating of -6.53, which is nearer to Albendazole, i.e., -6.79, ensuring that β-eudesmol has a strong binding fondness in between protein and ligand. Conclusion: From the examinations, a conclusion can be drawn that the anthelmintic activity of P. tomentosa root in both in vitro and insilico assays. The information sustains β-eudesmol to be a useful anthelmintic compound beneficial to future clinical examinations.

Key words: In-silico, Autodock 4.0, Pavetta tomentosa, β-eudesmol, Albendazole, ADME/T.