In vitro and in vivo Exploration of Super-magnetically Modulated Novel Parenteral Carrier: A Targeted Drug Delivery of Polymeric Carboplatin Nanosphere Capped with the Vitamin C to Treat Breast Cancer in Rats

Abstract:

Introduction: Super-magnetically modulated nanosphere of carboplatin (CPt) capped with vitamin C and uncapped Nano sphere of CPt were developed and evaluated for their use as a targeted drug delivery system. Objectives: The objective of the study was to encounter multiple problems such as to circumvent the snag caused by the larger molecules, increase the target ability of magnetic particle and synergize the impact of carboplatin with antioxidant effect. Methods: Particulate carriers for magnetic drug delivery were prepared by an emulsion-solvent evaporation method. The in vitro parameters such as drug entrapment efficiency, particle size analysis, Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), percentage magnetite content, swelling kinetics, in vitro magnetic responsiveness, in vitro drug release and kinetics study, in vitro antioxidant study were conducted followed by an in vivo anti- cancer study in rats to confirm the product efficacy or therapeutic benefits. Results: The maximum drug entrapment efficiency was found to be 88.93 % ± 0.77 at the higher concentration of polymers and considered to be good. SEM ensured spherical shape and coating of vitamin C, mean particle size and PDI value of F3 was found to be 150 nm and 0.251 respectively, DSC proved no significant interaction, maximum magnetite content was found in F3. Vitamin C coated carboplatin Nano sphere showed excellent in vitro antioxidant free radical-scavenging activity. Biphasic behaviour was not observed in the formulations F1-F3 however, F4-F6, displayed initial burst release followed by sustained release over a period of 24 h. The kinetic study suggested diffusion coupled with erosion release pattern. in vivo results showed that F-3 in the presence of the magnetic field had effectively suppressed breast cancer cells. Conclusion: The current study opens up new vistas in cancer treatment and established better in vitro - in vivo co-relationship under the diseased state.

Key words: Carboplatin, Ethyl cellulose, Magnetite, Vitamin C, Nanopshere.