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Published on:August 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(3s):s264-s272
Original Article | doi:10.5530/ijper.53.3s.96

Investigations on Apoptotic Activities of Cherry Laurel Extracts in HCT-116 Human Colon Carcinoma Cells

Authors and affiliation (s):

Bilal Çakir1,2, İbrahim Gülseren3,*

1İstanbul Sabahattin Zaim University, Halal Foods R and D Center, Halkalı, Küçükçekmece, İstanbul, TURKEY.

2Department of Biochemistry, Marmara University, Institute of Health Sciences, Faculty of Pharmacy, Başıbüyük, Maltepe, İstanbul, TURKEY.

3Department of Food Engineering, İstanbul Sabahattin Zaim University, Halkalı, Küçükçekmece, İstanbul, TURKEY.


Background: Cherry laurel fruits are characterized by their dark color and rich phenolic contents. Previously, phenolic extracts from cherry laurel varieties were shown to demonstrate anti-proliferative activities against cancerous cell lines. Aim: To characterize the phenolic compound content of cherry laurel varieties since their characterization is incomplete and determine their apoptotic potential. Methods: The phenolic compounds of fruit extracts from 3 different cherry laurel varieties were analyzed using an LC-MS/MS method. Using flow cytometry, apoptotic activities of cherry laurel extracts were studied against HCT-116 human colon carcinoma cells. Results: 14 different phenolic compounds were qualitatively determined, whereas for 5 compounds quantitation was possible. To the best of our knowledge, the presence of epicatechin, epigallocatechin, quercetin, quercetin-3-glucoside, procyanidin B2, delphinidine-3-O-rutinoside, delphinidine-3-Oglucoside, cyanidine-3-O-glucoside and cyanidine-3-O-rutinoside is being reported for the first time for these extracts. Although some dependence on phenolic profile and concentration was obvious, in most cases, a significant extent of apoptotic induction (40-60%) was observed against HCT-116 cells. Conclusion: Findings on anti-proliferative and apoptotic activity verified the anti-carcinogenic capabilities of cherry laurel extracts.

Key words: Cherry laurel, Phenolic compounds, Flow cytometry, Apoptosis, LC-MS/MS analysis, Anthocyanins.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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