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Published on:Jul 2012
Indian Journal of Pharmaceutical Education and Research, 2012; 46(3):197-205
Original Article | doi:NILL

Estimation of Stavudine, Lamivudine and Nevirapine by Chemometric UV Spectroscopic Method


Authors and affiliation (s):

Abhilasha Gupta, S.T. Bhagawati, B.S.Muddukrishna, Jagadish P.C and Krishnamurthy Bhat*

Manipal College of Pharmaceutical Sciences, Manipal- 576104.

Abstract:

A chemometric UV- spectrophotometric method of analysis was successfully developed for the simultaneous determination of stavudine, lamivudine and nevirapine in pharmaceutical formulation. The application of multivariate method to the obtained spectrophotometric data is a new idea for the simultaneous quantitative analysis of stavudine, lamivudine and nevirapine in samples. Multivariate calibration models were built from the raw PLS(Partial least square) method. Verification of the calibration carried out with the aid of prediction set of mixtures of these three drugs produced satisfactory result. The results also showed that for practical purposes, PLS model constructed was effective in their performance prediction. The precision study showed that % RSD was within the range of acceptable limits, the mean recovery (mean ± SD) was found to be 100.24 ± 1.23, 100.52 ± 1.52, 99.72 ± 1.57 and 100.23 ± 1.55, 99.66 ± 1.03, 99.15 ±1.39 for stavudine, lamivudine and nevirapine for two different batches of the formulation respectively. The good recoveries obtained in this case proved that, the proposed procedures could be applied efficiently for determination of studied drug simultaneously in their mixture as well as in the commercial dosage form with satisfactory precision and accuracy.

Keywords: Antiretrovirals, chemometric, UV spectroscopy, stavudine, lamivudine, nevirapine

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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