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Published on:April 2019
Indian Journal of Pharmaceutical Education and Research, 2019; 53(2s):s186-s192
Original Article | doi:10.5530/ijper.53.2s.65

EPR and UV-Vis Spectroscopic Studies of the Influence of Ultraviolet Irradiation on Antioxidant Interactions of Nystatin

Authors and affiliation (s):

Paweł Ramos*, Barbara Pilawa, Piotr Pepliński

School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice Department of Biophysics, Jedności, Sosnowiec, POLAND.


The changes of the free radical scavenging activity of nystatin after UV-irradiation were examined. The kinetics of the interactions of nystatin with free radicals was tested. The model DPPH free radicals were used. Free radicals were tested by an X-band (9.3 GHz) electron paramagnetic resonance spectroscopy. The EPR spectra of DPPH and DPPH in contact with non-irradiated and UV-irradiated nystatin were measured. Nystatin quenched the EPR spectra of DPPH free radicals as the result of the antioxidative character of the samples. The antioxidative character of non-irradiated and UV-irradiated nystatin was confirmed by the UV-Vis studies. Free radical scavenging activity decreased after UV-irradiation of nystatin and the EPR spectra of DPPH free radicals lower decreased by contact with UV-irradiated nystatin. The quenching of UV-Vis spectra were lower for UV-irradiated nystatin than for than with non-irradiated drug. It was pointed out that nystatin should not be storage under ultraviolet irradiation, because of decrease its antioxidative interactions. This studies confirmed usefulness of EPR spectroscopy and UV-Vis spectrophotometry to determine of the antioxidant character of drugs. These spectroscopic methods may be used to find the optimal storage conditions of drugs.

Key words: Nystatin, Antioxidant, UV-irradiation, EPR, UV-Vis.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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