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Published on:August 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4s):S47-S55
Original Article | doi:10.5530/ijper.52.4s.75

Synchronous Estimation of Glycopyrrolate and Formoterol in Bulk and Pharmaceutical Dosage Form by RP-HPLC Method


Authors and affiliation (s):

Kishor Kumar Erukulla, Suseem Sundaram Rengitham*

Department of Chemistry, VIT University, Vellore, Tamilnadu, INDIA.

Abstract:

Objective: The objective is to develop and validate a fundamental, specific, novel and exact RP-HPLC (Reverse phase-High performance Liquid chromatography) methodology for the synchronous determination of Glycopyrrolate and Formoterol in pharmaceutical dosage form. Methods and Materials: The column utilized was BDS C18 (250mm x 4.6 mm, 5μm) in isocratic mode, with mobile phase consist of phosphate buffer and acetonitrile (40:60 v/v). The buffer was made by adding 1 ml of Orthophosphoric acid in a 1000ml of volumetric flask and about 900ml of milli-Q water, degas to sonicate and lastly make up the quantity with water. The flow rate used was 1.0ml/ min and effluents were monitored at 241 nm. Results: The retention times of Glycopyrrolate and Formoterol are 2.290 min and 2.853 min, respectively. The linearity for Glycopyrrolate and Formoterol are 2.25-13.5μg/ml and 1.2-7.2μg/ml respectively. The recoveries of Glycopyrrolate and Formoterol were observed to be 99.17 to 100.66% and 98.36 to 100.79% respectively. Conclusion: The validation was performed for the proposed method and applied successfully for the determination of Glycopyrrolate and Formoterol. The method was found to be precise, accurate for the synchronous determination of Glycopyrrolate and Formoterol in pharmaceutical dosage form.

Key words: Glycopyrrolate, Formoterol, Validation, HPLC.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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