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Published on:August 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(4s):s23-s28
Original Article | doi:10.5530/ijper.52.4s.72

Simultaneous Determination of Some 5-Nitroimidazole Derivatives and Ciprofloxacin by High Performance Liquid Chromatography


Authors and affiliation (s):

Stanislav Bozhanov1*, Vania Maslarska1, Boyka Tsvetkova2, Lily Peikova2

1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.

2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav st., 1000 Sofia, BULGARIA.

Abstract:

Objective: An isocratic high performance liquid chromatographic method for simultaneous determination of Metronidazole, Tinidazole and Ciprofloxacin was developed. Method: Separation was achieved with a C18 (250x4.6 mm, 5 μm) column. Optimal chromatographic conditions were: mobile phase consisting of acetonitrile: 0.3% o-phosphoric acid modified with 0.1% triethylamine (20:80 v/v) at a flow rate of 0.7 mL min-1, at 30°C and wavelength of 300 nm. Results: The retention times were 4.92 min for Metronidazole, 8.78 min for Tinidazole and 9.76 min for Ciprofloxacin. The responses were linear (R2 = 0.9999) in the range of 12.5 – 100 μg mL-1. The limits of detection were 0.012 µg mL-1 for Metronidazole and Tinidazole and 0.04 µg mL-1 for Ciprofloxacin. The limits of quantification were 0.125 µg mL-1 for Metronidazole and Tinidazole and 0.4 µg mL-1 for Ciprofloxacin. The recovery (%) was within the range of 99.57 – 100.3% for all analytes. Conclusion: The method developed can successfully be used for routine analysis and quality control of Metronidazole, Tinidazole and Ciprofloxacin.

Key words: Metronidazole, Tinidazole, Ciprofloxacin, RP-HPLC, Validation, Quality control.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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