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Published on:16 Nov 2013
Indian Journal of Pharmaceutical Education and Research, 2013; 47(3):77-81
Pharmaceutical Research | doi:10.5530/ijper.47.3.12

Determination and Quantification of Ferulic acid in Ferula asafoetida by a Validated HPTLC Method


Authors and affiliation (s):

Joseph Kareparamban*, Pravin Nikam, Aruna Jadhav and Vilasrao Kadam

Department of Quality Assurance, Bharati Vidyapeeth’s College of Pharmacy, Sector-8, Belapur C.B.D., Navi Mumbai-400 614.

Abstract:

Phenolic acids are a group of compounds categorized as plant secondary metabolites widely distributed in plant kingdom and which furnishes with a number of applications. The oleo gum resin obtained from Ferula asafoetida commonly known as ‘HING’ possess ferulic acid which is one of a phenolic acid as an ingredient with a potent pharmacological activity. In the present study an attempt has been made to develop an HPTLC method for the quantitative estimation of ferulic acid from Ferula asafoetida. The method employed TLC aluminum plates precoated with silica gel 60F254 as stationary phase. Linear ascending development with toluene-ethyl acetate-formic acid in the ratio 60:40:10 as mobile phases were performed at room temperature (25 ± 2)ºC in a twin trough chamber saturated with mobile phase vapour. The system was found to give a compact spot for ferulic acid (Rf value of 0.48 ± 0.02). The detection was carried out at a wavelength of 319 nm. Linear regression analysis of calibration plot showed good co-relation between peak area and concentration with a co-relation coefficient r2 = 0.989 in the range of 200 ng/spot – 700 ng/spot. The developed method was validated for various validation parameters such as linearity, precision, accuracy as recovery, specificity, detection and quantification limits. The developed HPTLC method can be employed as a quality control tool for checking the purity of asafoetida resin powder, commercially available asafoetida and formulations comprising of asafoetida.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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