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Published on:June 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(3):426-436
Original Article | doi:10.5530/ijper.52.3.49

Antioxidant and Antiurolithiatic Efficacy of Aerva lanata (L) Fractions by in vitro and in vivo Screening Techniques


Authors and affiliation (s):

Basavaraj Mrutyunjay Dinnimath*1, Sunil Satyappa Jalalpure2

1Department of Pharmachemistry, KLE Academy of Higher Education and Research, College of Pharmacy, Belagavi, Karnataka, INDIA.

2Department of Pharmacognosy, KLE Academy of Higher Education and Research, College of Pharmacy, Belagavi and KAHER’S Dr.Prabhakar Kore’s Basic Science Research Center, Nehrunagar, Belagavi, Karnataka, INDIA.

Abstract:

Background: Aerva lanata (L) Juss. Ex. Schult. (Amaranthaceae) commonly known as Pashanabheda is used traditionally in Indian system of medicines for various diseases including urolithiasis. Objective: Screening of bioactivity guided fractions of Aerva lanata (L) for antioxidant and antiurolithiatic potency. Materials and Method: The dried hydro alcoholic extract (10%) of the whole plant was fractionated with different organic solvents like dichloromethane (fraction I), ethyl acetate (fraction II), n-butanol (fraction III). Based on preliminary phytochemical tests, only two fractions (II and III) were tested for antioxidant efficacy by DPPH Radical Scavenging assay and antiurolitiathic potentials on male Wistar albino rats by administering ethylene glycol (0.75% v/v) by oral route. Urine and blood serum were collected from all the tested animals and analyzed for the presence of calcium, oxalate, magnesium, phosphate, and BUN. Both kidneys were isolated and subjected to histopathological study. Results: Antioxidant assay has revealed that the tested fractions were equally potent as that of the standard drugs (BHT and Vitamin C). The treatment with ethyl acetate and n-butanol fractions significantly decreased the calcium, phosphate and BUN levels and increase in magnesium level in experimental rats (p<0.001). The urine analysis has also revealed significant (p<0.001) reduction in crystal size in the treated rats. Conclusion: The fractions (ethyl acetate and n-butanol) have shown significant antioxidant effect along with the Antiurolithiatic potency by reducing calculi through different pathways along with mild diuretic activity.

Key words: Pashanabheda, Ethylene glycol, Calcium oxalate, Antiurolithiatic activity, Antioxidant activity.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs