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Published on:January 2018
Indian Journal of Pharmaceutical Education and Research, 2018; 52(2):284-292
Original Article | doi:10.5530/ijper.52.2.33

Comparison of Mean Centering of Ratio Spectra Based Spectrophotometric Approach and HPLC Method for Quantitative Determination of Pirenoxine in the Presence of Methylparaben and Propylparaben

Authors and affiliation (s):

Hendri Wasito1,2, Sawanya Buranaphalin1, Lawan Sratthaphut3, Leena Suntornsuk1, Prapin Wilairat4,5 , Chutima Matayatsuk Phechkrajang1,4,6*

1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University; 447 Sri-Ayuthaya Road, Phayathai, Ratchathewi, Bangkok, THAILAND.

2Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University; Dr. Soeparno Road, Karangwangkal, Purwokerto, INDONESIA.

3Department of Health-Related Informatics, Faculty of Pharmacy, Silpakorn University; Nakornpathom Campus, 6 RajamankhaNai Road, Phrapathom Chedi, Muang, Nakorn Pathom, THAILAND.

4Flow Innovation-Research for Science and Technology Laboratories (FIRST Labs), Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND.

5National Doping Control Center, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok, THAILAND. 6Center of Excellence for Innovation in Drug Design and Discovery, Faculty of Pharmacy, Mahidol University, Bangkok, THAILAND.


Objective: The mean centering of ratio spectra method (MCR) was developed for determination of pirenoxine in the presence of methylparaben and propylparaben. Background: The UV spectrum of pirenoxine was suffered from spectra overlapping of methylparaben and propylparaben, the preservatives used in the eye drop formulation. Since, MCR method was introduced to overcome this limitation. Methods: The developed MCR method was performed using 39 synthetic mixtures of pirenoxine, methylparaben and propylparaben. The amplitudes at 320 nm of the second ratio spectra were used to construct a calibration model for pirenoxine. Performance characteristics of the method such as linearity, accuracy and precision, were calculated. A high-performance liquid chromatography (HPLC) method was also developed and validated. Then, two methods were used to determine a set of commercial eye drop samples for comparison. Results: The developed and validated MCR method was simple, rapid, accurate, and precise and could be applied to determine pirenoxine in eye drop samples. Measurement of pirenoxine in eye drop samples by MCR and HPLC methods were not significantly different (P-value = 0.21).

Key words: Pirenoxine, Mean centering of ratio spectra, HPLC.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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