Home | Articles
Published on:20 sep 2013
Indian Journal of Pharmaceutical Education & Research, 2013; 47(2):160-167
Research Article | doi:Not Applicable

Determination of Lamotrigine and Felbamate in Pediatric Plasma Samples by Reverse Phase High-performance Liquid Chromatography


Authors and affiliation (s):

Abstract:

A simple, sensitive, selective, fast and validated isocratic reversed-phase HPLC method for simultaneous determination of the new generation antiepileptic drugs, lamotrigine (CAS 84057-84-1) and felbamate (CAS 25451-15-4) in human plasma using spectrophotometric detection is presented in this paper. Lamotrigine, felbamate and the internal standard nevirapine (CAS 129618-40-2) were extracted from plasma (100μL) using liquid-liquid extraction under alkaline conditions into an organic solvent. Liquid chromatographic analysis was carried out on a Grace Vydac 5μm RP, 250mm ×4.6mm I.D. column, using a mixture of potassium dihydrogen phosphate buffer (15 mM, pH 3.2) and acetonitrile (70:30, v/v) as the mobile phase, at a flow rate of 1.0mL/min. The UV detector was set at 210nm. Retention times were 4.10, 5.30 and 6.70 min for lamotrigine, felbamate and I.S., respectively. Calibration curves were linear (mean correlation coefficient > 0.999 for both the analytes) over a range of 26.0-15000.0 ng/mL for lamotrigine and 230.0-80000.0 ng/mL for felbamate. Both intra and inter-assay precision and accuracy were lower than 8.17% for both the analytes. Absolute recoveries ranged between 94-97%. No interferences from either endogenous plasma components or other commonly prescribed antiepileptic drugs and analgesics were observed. This reliable method can be applied to pharmacokinetic investigations, bioequivalence, overdose, drug-drug interaction and therapeutic drug monitoring studies where small sample sizes are available e.g. pediatrics samples.

 




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the Latest Release of Journal Citation Reports (source: Web of Science Data).

 

Impact Factor® as reported in the 2023 Journal Citation Reports® (Clarivate Analytics, 2023): 0.8

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs