Determination of Some Flavonoids and Antimicrobial Behaviour of Some Plants' Extracts

Abstract:

C. sativa, C. intybus, L. stoechas, V. officinalis and G. glabra plants were extracted by using 65% ethanol to isolate their active constituents. The antimicrobial activities of extracts were investigated against 15 microorganisms by using the disk diffusion method, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) and MFC (Minimal Fungicidal Concentration) tests. Furthermore, the presence of eight flavonoids were analysed by using HPLC. It was found that C. sativa is active against C. albicans, E. faecalis, S. enteritidis and S. typhimurium with MIC values of 26.02 μg/mL, 13.01 μg/mL, 416.25 μg/mL and 832.50 μg/mL respectively, where C. intybus is active against C. albicans and E. faecalis, with MIC values of 13.01 μg/mL and 6.50 μg/mL, respectively. On the other hand, L. stoechas and V. officinalis were observed to be active against only S. enteritidis with MIC values of 52.03 μg/mL and 26.02 μg/L respectively, where G. glabra was active against only E. faecalis, with a MIC value of 52.03 μg/mL. The extracts of plant samples showed antibacterial activity against tested microorganisms at different levels. But the activities against C. albicans and E. faecalis is noteworthy. The flavonoids were determined at different amounts in extracts.

Key words: Soxhelet Extraction, Antimicrobial Activity, Flavonoid, MIC, Disc Diffusion.