Home | Articles
Published on:July 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(4):729-739
Original Article | doi:10.5530/ijper.51.4.107

Attenuation of Oxidative Stress and Hepatotoxicity Induced By D–Galactosamine by a Polyherbal Formulation Ambrex–in vivo and in vitro Studies


Authors and affiliation (s):

Anila Raslin1, Sekar Sathiya2, Chidambaram Saravana Babu2, Johanna Rajkumar1*

1Department of Biotechnology, Rajalakshmi Engineering College,Thandalam, Chennai - 602105, Tamilnadu, INDIA.

2Centre for Toxicology and Developmental Research, Sri Ramachandra University, Porur, Chennai - 600110, Tamilnadu, INDIA.

Abstract:

Objective: To evaluate the hepatoprotective effect of Ambrex, a polyherbal formulation against D–galactosamine (D-GLN) induced hepatotoxicity in Swiss albino mice as well as in Chang liver cell lines. Materials and Methods: Ambrex was orally administered for a period of 7 days at dose levels of 250 and 500 mg/kg b.wt. D-GLN (250 mg/kg b.wt, i.p) was administered 24h prior to sacrifice the animals. The protective effect of Ambrex was evaluated by measuring plasma levels of aspartate transaminase (SGOT), alanine transaminase (SGPT), alkaline phosphatase (ALP), γ–glutamyltransferase (γGT) and total bilirubin. Its effect on antioxidants such as superoxide dismutase (SOD), catalase (CAT) and reduced glutathione (GSH) and lipid peroxide(LPO) was also determined. Histopathological evaluation of liver tissues was carried out. Results: Data revealed that Ambrex was able to restore the levels of antioxidants such as SOD, Catalase, and Glutathione to near normal and reduced the elevated plasma levels of SGOT, SGPT, ALP, γ – GT and total bilirubin. It also inhibited the formation of hepatic malondialdehyde induced by D - GLN. In vitro studies revealed that Ambrex protected D- GLN induced hepatotoxicity (30μM/ ml) at dose levels of 5, 50 and 500ng/ml. Further, mRNA expression also illustrated that Ambrex inhibited the over expression of Bax, Caspase 3, TNF–α, IL–2 and CYP–450. A substantial decrease in the mRNA expression of anti-apoptotic marker Bcl2 was also observed. Conclusion: Results suggest that Ambrex has appreciable hepatoprotective effect which was evident from both in vivo and in vitro results.

Key words: Ambrex, D- Galactosamine, Hepatotoxicity, Chang Cells, Antioxidants.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
2 + 18 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
4 + 4 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs