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Published on:June 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(3):407-411
Original Article | doi:10.5530/ijper.51.3.68

Sub-cellular localization of Tetrodotoxin in the Tissues of the Pufferfish Takifugu Oblongus


Authors and affiliation (s):

Indumathi S M and Samanta Sekhar Khora*

Department of Integrative Biology, School of Biosciences and Technology, VIT University, Vellore – 632014, Tamil Nadu, INDIA.

Abstract:

Objective: The prime objective of this study is to determine the origin of Tetrodotoxin in the pufferfish Takifugu oblongus. Methods: Ultracentrifugation was performed to separate the tissues into various cellular fractions. Mouse bioassay was done to analyse the toxicity of different fractions from skin, liver and ovary of T. oblongus. The toxic fractions were subjected to HPLC-UV detection, to detect the presence of tetrodotoxin and quantify it. Results: Cytosolic fractions of skin, liver and ovary were extremely toxic and the presence of tetrodotoxin was proved on comparison with the retention time of standard toxin. Conclusions: These results suggest that the origin of tetrodotoxin could be endogenous or biosynthesized by the pufferfishes themselves. Clarifying the contradictions about the origins of TTX in puffers would allow researchers to accurately identify its role and toxicogenesis of the organisms which possess tetrodotoxin.

Key words: Takifugu oblongus, Tetrodotoxin, Ultracentrifugation, Mouse Bioassay, HPLC.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs