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Published on:March 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(2):337-342
Original Article | doi:10.5530/ijper.51.2.40

Validation of Capsaicin in Indian Capsicum Species Through RP-HPLC


Authors and affiliation (s):

Mrinmoy Nag1, Joydeb Chanda1, Rajarshi Biswas1, Naif Abdullah Al-Dhabi2, V. Duraipandiyan2, Pratim Banerji3 and Pulok K. Mukherjee1*

1School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata-700032, INDIA.

2Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, Riyadh-11451, SAUDI ARABIA.

3Ulysses Research Foundation, 125, Rashbehari Avenue, Kolkata 700029, West Bengal, INDIA.

Abstract:

Capsaicin is a pungent capsacinoid which differs in capsicum fruit within the species and the cultivars due to its topographical diversity. Here, a comparative estimation of capsaicin in seven varieties of capsicum has been performed by RP-HPLC method. The method was carried out in reverse phase C18 column using acetonitrile and water (1% acetic acid) as mobile phase (65:35 v/v), at the flow rate of 1ml/min. The λmax was detected at 230 nm. The calibration curves were linear in the concentration range of 1-80 μg/ml. The comparative study revealed that the capsaicin content was highest (3.12% w/w) in Capsicum annuum Cayenne whereas Capsicum cardenasii contains the lowest (0.85% w/w). The % RSD of precision and recovery was found to be < 2%, which confirms high repeatability of the method. This method can be commercialized at industrial level for ensuring the highest quality of capsicum used as raw material.

Key words: Ayurveda, Capsaicin, RP-HPLC, Method validation, Capsicum.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs