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Published on:March 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(2):288-294
Original Article | doi:10.5530/ijper.51.2.34

D-Tagatose production by Lactococcus lactis NZ9000 Cells Harboring Lactobacillus plantarum L-arabinose Isomerase


Authors and affiliation (s):

Yao Zhang, Yanli Fan, Haijie Hu, Haixu Yang, Xuegang Luo, Zhongyuan Li, Hao Zhou, Wenjian Ma, Yajian Song*, Tongcun Zhang

Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Laboratory, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, People’s Republic of CHINA.

Abstract:

D-tagatose is a functional sweetener present in medicine, food, and dairy products and with broad market prospects, and L-arabinose isomerase gene (araA) can mediate the bioconversion of D-galactose into D-tagatose. In this study, a Lactococcus lactis NZ9000 strain harboring exogenous L-arabinose isomerase was constructed to produce D-tagatose. Lactobacillus plantarum CGMCC 8198 exhibits L-arabinose isomerase activity and its genome has been sequenced. The araA gene of Lactobacillus plantarum CGMCC 8198 encoding L-arabinose isomerase was identified by sequence analysis and was successfully cloned and expressed in Lactococcus lactis NZ9000. The D-tagatose production by the whole cell of the recombinant strain was optimized. The optimal condition for conversion reaction was at 50°C, pH 7.0 and with 300 mmol/L Mn2+ and 60 g/L galactose added. The D-tagatose yield and conversion rate at the optimal condition was determined and reached 40.2 g/L and 67%, respectively.

Key words: D-tagatose, L-arabinose isomerase, Lactococcus lactis NZ9000, Lactobacillus plantarum, Galactose.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs