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Published on:March 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(2):270-279
Original Article | doi:10.5530/ijper.51.2.32

Alginate-chitosan Coated Lecithin Core Shell Nanoparticles for Curcumin: Effect of Surface Charge on Release Properties and Biological Activities


Authors and affiliation (s):

Lokesh Pathak1, Tripathy Amrutanand2, Yadvendra Agrawal1

1Institute of Research and Development, Gujarat Forensics Sciences University, Gandhinagar, Gujarat, INDIA.

2Al-Ameen College of Pharmacy, Hosur Road, Bangalore-560027, Karnataka, INDIA.

Abstract:

Alginate-chitosan coated lecithin nanoparticles loaded with Curcumin have been prepared by layer deposition of alginate on lecithin/chitosan nanoparticles and were characterized. The prepared nanoparticles were physicochemically characterized to ensure alginate coating. The encapsulation efficiency and loading of the active nutraceutical were assessed using spectrophotometric measurements. The biological activities of the prepared nanoparticles were assessed by membrane stabilization effect of the nanoparticles on goat RBCs under hypotonic and thermally induced conditions. The biocompatibility of blank nanocarriers was assessed by performing In vitro cytotoxicity studies on HEK cell lines. The release profile of curcumin from alginate uncoated and alginate coated nanoparticles were compared to analyze effect of surface charge of the particles on studied bioactivities. It was found that uniformly sized alginate coated nanoparticles were formed with negative surface charge. It was also observed that there was no significant difference due to surface charge of the nanoparticles on cytotoxicity and membrane stabilization activity. However alginate coating offered more controlled release of the curcumin in acidic conditions suggesting that these alginate coating imparted enteric coating character.

Key words: Layer deposition, Membrane stabilization, MTT assay, Membrane stabilization, Biocompatibility.

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs