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Published on:30th Jan 2014
Indian Journal of Pharmaceutical Education and Research, 2013; 47(4):65-72
Pharmaceutical Research | doi:10.5530/ijper.47.4.9

High-performance Liquid Chromatographic Determination of Paracetamol, Propyphenazone, and Caffeine in Pharmaceutical Formulations

Authors and affiliation (s):

Kuldeep Delvadiya1, Prachi Kabra1, Ritu Kimbahune2*, Nikunj Patel1 and LVG Nargund1
1Department of Quality Assurance, Nargund College of Pharmacy, BSK III stage, Bangalore - 560 085, India
2Department of Quality Assurance, Al-Ameen College of Pharmacy, Hosur Rd, Near Lal Bagh Bangalore - 560 027, India


This study proposes a liquid chromatography (RPHPLC) method for quantitative analysis of the most widely prescribed combination of Paracetamol, Propyphenazone and Caffeine in tablet dosage form in presence of Rasagiline as an internal standard. The chromatography was performed on a Gracesmart C18 column (5μm, 250mm×4.6mm i.d.) using the mixture of water and 2-propanol in the ratio 80:20 v/v as mobile phase. The pH of aqueous phase was adjusted to 3.0 with 1% o-phosphoric acid and a flow rate was maintained at 1.5ml/min. The analytes were detected by measuring the absorbance at 210nm. The chromatographic analysis time for each sample was kept about 20min as Rasagiline, Paracetamol, Caffeine and Propyphenazone were eluted at 1.63, 2.36, 3.17 and 11.22 min respectively. The calibration plots were obtained between 3–90μg/ml for Paracetamol, 1.5–45μg/ml for Propyphenazone and 0.5–15μg/ml for Caffeine. The method was validated in accordance with ICH norms and method was found to be specific, accurate, precise and easy to use for routine analysis.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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