Home | Articles
Published on:January 2017
Indian Journal of Pharmaceutical Education and Research, 2017; 51(1):51-58
Original Article | doi:10.5530/ijper.51.1.8

Morus rubra Extract Induces G1 Cell Cycle Arrest and Apoptosis in Human Lung and Prostate Cancer Cells


Authors and affiliation (s):

Ibrahim Turan1,2, Selim Demir3*, Kagan Kilinc1, Yuksel Aliyazicioglu4, Ahmet Alver4, Sema Misir4,5, Serap Ozer Yaman4, Kubra Akbulut4, Ahmet Mentese6, Orhan Deger4

1Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University, 29100, Gumushane

2Medicinal Plants, Traditional Medicine Practice and Research Center, Gumushane University, 29100, Gumushane

3Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080, Trabzon

4Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon

5Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University, 58140, Sivas.

6Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.

Abstract:

Objective: Cancer is one of the most deadly types of disease and evasion from apoptosis and unstoppable cell proliferation are accepted as its distinctive features. Many studies have evaluated the cytotoxic effect of different Morus species but, there is no study about cytotoxic effect of Morus rubra. In this study we aimed to evaluate phenolic composition, antioxidant properties and cytotoxic effect of acidified dimethyl sulfoxide extract of M. rubra (AMRE). Method: Antioxidant properties, phenolic composition and cytotoxic effect of AMRE were determined using spectrophotometric methods, HPLC, and MTT assay, respectively. Then, mechanisms of cytotoxic effect of AMRE on human prostate (PC-3) and lung (A549) cancer cells were examined in regard to cell cycle, apoptosis and mitochondrial membrane potential using flow cytometric methods. Results: Total phenolic content and reducing power values were 11.9 mg gallic acid equivalents and 42.9 mg trolox equivalents per g sample, respectively. Ascorbic and gallic acid were detected in AMRE as major antioxidant compounds. We determined that AMRE increased cell cycle arrest at G1 phase and exhibited apoptotic features via decreasing mitochondrial membrane potential in both prostate and lung cancer cells. Conclusion: These findings demonstrate that M. rubra extract can affect the behavior of human prostate and lung cancer cells in vitro conditions, and this effect now needs to be investigated in vivo.

Key words: Apoptosis, Cell Cycle, Cytotoxicity, Lung Neoplasms, Morus rubra, Prostate Neoplasms.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
5 + 12 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

User login

CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
3 + 1 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs