Conventional extraction techniques like soxhlet extraction and reflux extraction and novel extraction techniques like Supercritical Fluid extraction (SFE), Ultrasonic Assisted Extraction (UAE) and microwave assisted extraction method (MAE) were used for the extraction of Convolvulus Pluricaulis. The scopoletin content in the extracts obtained using different extraction techniques was estimated using pre validated HPTLC method. The conventional and novel extraction techniques were compared on the basis of various parameters like extraction time, organic solvent consumption and extraction yield. The novel extraction techniques were found to be better than conventional methods as they require less time, consume less solvent and give higher yields of scopoletin. From amongst the novel extraction techniques used Microwave assisted extraction was found to give highest yield of scopoletin.
Key words: Scopoletin, Ultrasound assisted extraction, Supercritical fluid extraction, Microwave assisted extraction.