Enhancement in Cortical Arousal by Caffeine Salicylate in Experimental Animal Models

Abstract:

Objective: It is aimed to evaluate contribution of two molecules of Caffeine salicylate, a combinatorial product, in the CNS stimulant, anti-inflammatory and an analgesic property in experimental animal models. Material and Methods: Caffeine salicylate is synthecised by one step reaction from caffeine and salicylic acid. Actophotometer and open field test were used for the CNS stimulant activity whereas formalin induced rat paw edema and tail immersion test were used to evaluate anti-inflammatory and analgesic properties in experimental animals. Results: The locomotor activity was increased from 37.1% for caffeine to 49.24% for caffeine salicylate. In open field test; number of crossing and rearing were increased from 42.34 % to 54.89% and 17.26% to 27.87% respectively. The % inhibition on rat paw edema 16.40% and 18.75% with caffeine salicylate as against diclofinac 51.0% and 62.5% at 60 and 120 min. respectively. The tail withdrawal time was prolonged by 25% with caffeine salicylate as against 78.88 % with pentazocine in wistar rats. The results of all experimental animal models were statistically significant. Conclusion: The enhancement in the cortical arousal by caffeine salicylate over caffeine may be due to contribution of antioxidant property of salicylate besides adenosine antagonism and mobilization of intracellular calcium induced dopamine release by caffeine portion of caffeine salicylate. The mild analgesic and anti-inflammatory activities of caffeine salicylate may be due to inhibition of prostaglandin synthesis and an antioxidant properties of salicylate portion of caffeine salicylate.

Key words: Caffeine salicylate, combinatorial, cortical arousal, adenosine, antioxidant.