Home | Articles
Published on:August 2021
Indian Journal of Pharmaceutical Education and Research, 2021; 55(3):756-764
Original Article | doi:10.5530/ijper.55.3.148

Exploring the Neuroprotective Effects of Mechanosensitive Channel Blocker, GsMTX4 in Intracerebral Hemorrhage Model in Rats


Authors and affiliation (s):

Wenyan Liu1, Guiyu Zhang1, Jin Wang1, Jing Liu1, Yu Yuan1, Jun Hong1, Yi Ding2,*

1Department of Neurosurgery, Tangshan Gongren Hospital, Tangshan, Hebei, CHINA.

2Surgical Intensive Care Unit, Chongqing Jiangjin Central Hospital, Chongqing, CHINA.

Abstract:

Aim: The current study explored the usefulness of a mechanosensitive channel blocker, GsMTX4 in intracerebral hemorrhage (ICH)-associated deleterious effects along with the possible mechanisms. Materials and Methods: Type VII collagenase was administered in the right basal ganglia using stereotactic apparatus to induce ICH in rats. The ICHassociated injury was assessed using corner turn and forelimb placement tests (behavioral test); Evans blue extravasation test (blood-brain barrier damage), brain edema, apoptotic markers (caspase-3 and Bcl-2 levels). The levels of TNF-α (neuroinflammation), reactive oxygen species, and Brain-derived neurotrophic factor (BDNF) were also measured in ICH-subjected rats. GsMTx4, as a mechanosensitive channel blocker, and ANA-12, as a selective BDNF receptor blocker were employed as pharmacological agents. Results: Administration of GsMTx4 attenuated ICH-associated behavioral changes, preserved blood-brain barrier, prevented brain edema, and decreased apoptosis. GsMTx4 also decreased neuroinflammation and oxidative stress, while it increased the levels of BDNF. Moreover, administration of ANA-12 attenuated the neuroprotective effects of GsMTx4 suggesting that BDNF may be important in inducing neuroprotective effects of GsMTx4. Conclusion: GsMTx4 exerts neuroprotective effects in intracerebral hemorrhageassociated deleterious effects, which may be possibly due to an increase in BDNF levels along with a decrease in neuroinflammation and oxidative stress.

Key words: Neuroinflammation, Oxidative stress, Intracerebral hemorrhage, Mechanosensitive channels, BDNF.

Downloads

 




 

Impact Factor

IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

Impact Factor® as reported in the 2018 Journal Citation Reports® (Clarivate Analytics, 2019): 0.425

The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

DOI HISTORY

IJPER uses reference linking service using Digital Object Identifiers (DOI) by Crossref. Articles from the year 2013 are being assigned DOIs for its permanent URLs