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Published on:1 January, 2012
Indian Journal of Pharmaceutical Education and Research, 2012; 46(1):70-74
Research Article | doi:Nill

Studies on Production of 6-Aminopenicillanic Acid by Free and k- Carrageenan Immobilized Soil Bacteria

Authors and affiliation (s):

Ashoke Kumar Dolui*, (Late) Sitikantha Sahana and Atul Kumar

Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, 786004, Assam, India


In the present study different bacterial samples from the soil of different places of Dibrugarh were isolated and incubated in presence of penicillin G (2 mg/ml) for 48 hours at 37°C for biotransformation to 6-amino penicillanic acid which was detected by Thin Layer Chromatography (TLC). Among 15 isolated bacterial samples, two gram positive bacterial samples designated as AKDD-7 and AKDD-9 produced 6-APA from penicillin G. Assessment of production of 6-APA was carried out by two selected samples separately in free and k-Carragennan immobilized state through HPLC in first stage after incubation in penicillin G (2 mg/ml) for 48 hours. Reusability of immobilized cells was found successful for production of 6-APA from penicillin G after one-day cold-preservation and another seven days' cold preservation in the second and third stage respectively.

Keywords: Penicillin G, 6- Aminopenicillanic acid, Cell immobilization, Bioconversion, HPLC assessment, k-Carragennan.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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