Columns in Pharmaceuticals: For Primers

Abstract:

Chromatographic processes have a crucial role in the manufacturing of pharmaceuticals, bio-pharmaceuticals, polypeptides, proteins, enzymes and antibodies. They are apprehended, purged and elegant by size exclusion, ion exchange, reverse phase, hydrophobic interaction or the affinity chromatographic processes. The key to the proper selection of column is the knowledge of the principles of the chromatographic process (HPLC, Ion exchange, size exclusion, gas chromatography, UPLC). A Chromatographic column is a tool used for the separation of various chemical compounds in mixtures. A chromatographic column comprises the stationary phase, letting the mobile phase to go from it. Chromatography columns of the various classes are used in gas and liquid chromatography. Make certain robustness and reproducible performances of column, columns are needed to be qualified in an acceptable fashion before each use. This article describes the column specification and enables the reader or industrial primers to make the sense of the many parameters in details such as particle size, dimensions, surface area, pore size, carbon load which is used to describe HPLC columns, ion-exchange chromatography columns, size exclusion columns, chiral columns, gas chromatographic columns and UPLC chromatographic columns with application.

Key words: Column, Specifications, HPLC, Size exclusion, Gas chromatographic, UPLC, Bonded Phase.