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Published on:August 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(3):790-797
Original Article | doi:10.5530/ijper.54.3.131

Development and Validation of Stability Indicating Method for Estimation of Buparvaquone by Forced Degradation Studies


Authors and affiliation (s):

Shaikh Tanveer Kutubuddin1, Shelke Sushil Suresh1, Kakde Rajendra Baliram1,*, Lalatsa Aikaterini2

1Department of Pharmaceutical Sciences, RTMNU Nagpur, Maharashtra, INDIA.

2School of Pharmacy and Biomedical Sciences, University of Portsmouth, UK.

Abstract:

Aim: A lucid, rapid and precise stability-indicating method was developed by using HPLC for the estimation of Buparvaquone in bulk as well as pharmaceutical dosage form by forced degradation studies. Materials and Methods: Princeton C18 column (4.6 × 150 mm, 5μ) and mobile phase containing 1% glacial acetic acid and acetonitrile in the proportion of 5:95 v/v was used throughout the study. The flow rate was 0.9 ml/m and the detecting wavelength was kept as 251 nm using the PDA detector. Results: The retention time of Buparvaquone was found to be at 8.6 ± 0.5 m. The method developed was validated, as stated in ICH Q2 (R1) guidelines. It was found to be linear within concentration ranging from 2-20 μg/ml having a correlation coefficient 0.999 and other parameters are also under permissible limits. Conclusion: Buparvaquone was exposed to different stress conditions like acidic, basic, neutral, thermal, peroxide and also photolytic. Amongst all, the drug was found to be more degraded under basic as well as peroxide conditions.

Key words: Buparvaquone, Forced degradation, Validation, RP-HPLC, Stability.

 




 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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