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Published on:March 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(2):440-447
Original Article | doi:10.5530/ijper.54.2.50

LC-MS/MS Determination and Pharmacokinetics Study of Apremilast after Oral Administration in Rabbits

Authors and affiliation (s):

Tammisetty Mohan Rao1,*, Challa Balasekhara Reddy2, Puttagunta Srinivasa Babu3

1Department of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, INDIA.

2Department of Pharmaceutical Analysis, Vagdevi College of Pharmacy, Gurajala, Guntur, Andhra Pradesh, INDIA.

3Department of Pharmaceutics, Vignan Pharmacy College, Vadlamudi, Guntur, Andhra Pradesh, INDIA.


Objectives: A selective and reproducible method has been optimised for evaluation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS. Methods: The analytical technique was implemented to quantify the apremilast in rabbit plasma samples with Apremilast-D5 as deuterated internal standard. The chromatographic separation tuned with 10mM Ammonium Acetate Buffer (pH: 4.0): Methanol: Acetonitrile, (20:40:40%, v/v/v) using the CORTECS C18, 2.7 μ.m, 4.6 m.m X 150 m.m analytical column with analysis time four minutes. The flow of mobile phase through column is 0.5 m.L/min. The mass spectrometric ions of Apremilast and Apremilast-D5 obtained were m/z 461.5→257.1 and 466.5→257.1. Results: The curve indicates correlation coefficient (r2) was superior than 0.998 with linear range of 0.03-48.0 n.g/m.L. The developed method was tuned to apply efficaciously for analyzing the pharmacokinetic parameters of Apremilast in rabbit plasma samples. Conclusion: An accurate and reproducible novel method was fabricated for estimation of Apremilast in rabbit biological matrices by UPLC-ESI-MS/MS will be used for regular analysis and appropriate for therapeutic drug monitoring.

Key words: Apremilast, Rabbit plasma, UPLC-ESI-MS/MS, Bio-analysis, Pharmacokinetic, Therapeutic drug monitoring.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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