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Published on:March 2020
Indian Journal of Pharmaceutical Education and Research, 2020; 54(2):397-402
Original Article | doi:10.5530/ijper.54.2.45

Biochemical Investigation of the Protective Effect of Thiamine Pyrophosphate on Methotrexate-Induced Gastrotoxicity in Rats

Authors and affiliation (s):

Irfan Cinar1, Mine Gulapoglu2, Halis Suleyman3,*

1Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, TURKEY.

2Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, TURKEY.

3Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yıldırım, Erzincan, TURKEY.


Aim: The aim of the study is to investigate the protective effect of thiamine pyrophosphate on methotrexate- induced gastrotoxicity biochemically in rats and to compare it with thiamine. Methods: Rats were divided into four groups as sham (SG), thiamine+ methotrexate (TAMTX), thiamine pyrophosphate+methotrexate (TPMTX) and Methotrexate (MTX) groups. 5 mg/kg methotrexate was administrated to 24 hrfasted rat groups by oral gastric tube except sham rats. 20 mg/kg thiamine was injected to TAMTX group and 20 mg/kg thiamine pyrophosphate was injected to TPMTX group i.p. 5 min before the methotrexate administration. Equal amount distilled water was injected i.p. to SG and MTX groups. 6 hr after the methotrexate administration, the rats were sacrificed with high dose thiopental anesthesia (50 mg/kg). The stomachs of the rats were extracted and MDA, MPO, GSH and SOD analyzes were performed. Results: Biochemical results were evaluated by comparing among groups. While tGSH and MDA levels and MPO and SOD activities were 4.2±0.6, 10.0±0.9, 7.4±0.8, 5.2±0.9 μmol/g in MTX group, these parameters were 11.0±1.0, 3.4±0.4, 3.3±0.5, 13.2±1.1 μmol/g in SG group in the gastric tissue. These parameters were measured as 4.8±0.8, 8.7±0.8, 5.9±0.5, 6.1±0.8 μmol/g in TAMTX group and 10.8±0.8, 4.0±0.5, 3.6±0.4, 10.5±0.9 μmol/g in TPMTX group. Conclusion: The results showed that thiamine pyrophosphate prevented methotrexate induced oxidative damage in the gastric tissue, but thiamine could not prevent.

Key words: Gastrotoxicity, Methotrexate, Thiamine, Thiamin pyrophosphate, Ulcer.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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