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Published on:December 2019
Indian Journal of Pharmaceutical Education and Research, 2020; 54(1):206-212
Original Article | doi:10.5530/ijper.54.1.24

Formulation and Evaluation of Antibacterial Topical Gel of Doxycycline Hyclate, Neem Oil and Tea Tree Oil

Authors and affiliation (s):

Vishwanath Abasaheb Borse*, Avinash Balasaheb Gangude, Anil Bhavrao Deore

K. B. H. S. S. T’s. Institute of Pharmacy, Bhaygaon Road, Malegaon, Nashik, Maharashtra, INDIA.


Purpose: To formulate novel topical antibacterial gel containing Doxycycline Hyclate, Tea tree oil and Neem oil to evaluate their antibacterial activity. Methods: Formulation excipients were selected using Drug-excipients compatibility study. Prepared gel was subjected to various evaluation parameters like pH, viscosity, spreadability, homogeneity, drug content uniformity, in-vitro drug diffusion and antibacterial activity. Results: Prepared topical gel of doxycycline Hyclate was shown pH range 5.3±0.010 to 6.7±0.015, viscosity range 386±28.70 to 680±50.40 cp, spreadability range 18.14±0.98 to 28.13±2.18 g.cm/s and homogeneous. Prepared gel was shown uniformity in drug content which ranges from 94.41±0.61 to 97.44±0.69%, zone of inhibition ranges 13.66±1.69 to 32.00±1.63 mm for Staphylococcus aureus, 04.00±1.63 to 12.33±0.94 mm for Pseudomonas aeruginosa and 11.66±0.94 to 33.33±1.24 mm for Propionibacterium acne. Prepared gel formulations were shown the negligible amount of drug diffusion, not more than 3.22±0.04% was shown the non-fickian type of diffusion through the egg membrane. Conclusion: Formulation F8 was shown better antibacterial activity as compared to its other formulations. As the concentration of neem oil and tea tree oil was increased from 0.5 to 3% and 0.5 to 2% respectively in the gel, antibacterial activity was synergistically improved against Staphylococcus aureous, Pseudomonas aeruginosa and Propionibacterium acne. The prepared gel formulation was found useful for topical application due to its negligible diffusion, good spreadability, neutral pH, low viscosity and no irritation on human skin.

Key words: Doxycycline Hyclate, Neem oil, Tea tree oil, Drug diffusion study, Antibacterial activity.



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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.


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