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Published on:December 2019
Indian Journal of Pharmaceutical Education and Research, 2020; 54(1):166-174
Original Article | doi:10.5530/ijper.54.1.20

Eco-friendly Derivative UV Spectrophotometric Methods for Simultaneous Determination of Diclofenac Sodium and Moxifloxacin in Laboratory Mixed Ophthalmic Preparation


Authors and affiliation (s):

Muhammad Shahzad Chohan1, Rafea Elamin Elgack Elgorashe2, Abdulmalek Ahmed Balgoname3, Mahesh Attimarad3,*, Nagaraja SreeHarsha3, Katharigatta Narayanaswamy Venugopala3,4, Anroop Balachandran Nair3, Shinu Pottathil1

1Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Hofuf, Al-Ahsa, SAUDI ARABIA.

2Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, SAUDI ARABIA.

3Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Hofuf, Al-Ahsa, SAUDI ARABIA.

4Department of Biotechnology and Food Technology, Durban University of Technology, Durban, SOUTH AFRICA.

Abstract:

Background: Diclofenac Sodium (DCL) and Moxifloxacin HCl (MOX) were simultaneously used after cataract surgery to reduce the post-operative inflammation and to control infection respectively. Objectives: Three simple, accurate, eco-friendly and reproducible UV spectroscopic methods were established for concurrent determination of diclofenac sodium and moxifloxacin in ophthalmic preparation without prior separation. Methods: The first technique was established on the measurement of a peak amplitude of the first derivative spectra at the zero-crossing wavelength of one analyte. The second method was the determination of peak amplitude difference between peak and trough of ratio spectra. The third method involves the measurement of the peak amplitude of the first derivative of ratio spectra. Water has been used as a solvent. Results: The analytes exhibited good linearity in the range of 1 -15 μg/mL for DCL and 1-18 μg/mL for MOX with excellent correlation coefficient (r2>0.999). Low percent relative standard deviation confirmed the precision of the methods. Excellent recovery with low percent relative error proved the accuracy of the methods. The specificity of the methods was evaluated by analyzing the laboratory prepared solutions of DCL and MOX. Conclusion: Proposed three techniques were effectively utilized for the simultaneous determination of DCL and MOX from ophthalmic preparation. The outcomes of the proposed procedures were compared with the earlier described methods and no statistical difference was found between the methods in terms of accuracy and precision.

Key words: Diclofenac sodium, Moxifloxacin, Ophthalmic preparation, Ratio derivative spectroscopy, Validation.

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IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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