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Published on:December 2019
Indian Journal of Pharmaceutical Education and Research, 2020; 54(1):85-94
Original Article | doi:10.5530/ijper.54.1.10

Antiproliferative Effect of Crude Venom from Conus virgo on Human Lung Cancer Cell Line and Toxicity Assessment on Adult Zebra Fish (Danio rerio)


Authors and affiliation (s):

Dhana Sekaran Ganesan, Mohamed Asik Raj Mohamed, Pema Lhanzin, Kathirvel Neelan, Lakshmanan Vadakkuvaselvi, Sellamuthu Subramani, Arulvasu Chinnasamy*

Unit of Animal Tissue Culture and Aquaculture, Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamilnadu, INDIA.

Abstract:

Background: For the past two decades, few conotoxins entered in clinical trial and some are available for pain relieving as well as for treatment of neurological disorders. Aim: The present investigation was made to elucidate the antiproliferative effect of crude venom from Conus virgo against human lung adenocarcinoma cells. Acute toxicity was determined on adult Zebra fish. Methods: Cytotoxicity was assessed by MTT assay where the DNA damage was carried out by DNA fragmentation method and the in vivo toxicity was assessed by OECD guidelines. Results: The cell viability was found to decrease and the cell lost their morphological integrity with increasing concentration (10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 μg/ml) with an IC50 concentration of 74.69 μg/ml. The nuclear integrity and DNA fragmentation assay revealed the induction of DNA damage which was observed at 5 h. Further acute toxicity study was made, the LD50 was found at a concentration of 96.393μg/ml. The crude venom affects the locomotory activity of adult Zebra fish and induces the paralytic activity at 50, 75, 100 and 125 μg/ml concentrations. Histology of brain tissue indicated slight variations in the pallium. Conclusion: From the investigation, it was inferred that C. virgo venom could be a potential candidate for cancer chemotherapy.

Key words: A549, Conus virgo, Cone snail, Zebra fish, Acute toxicity.

 

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IJPER - An Official Publication of Association of Pharmaceutical Teachers of India is pleased to announce continued growth in the 2019 Release of Journal Citation Reports (source: 2018 Web of Science Data).

 

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The Official Journal of Association of Pharmaceutical Teachers of India (APTI)
(Registered under Registration of Societies Act XXI of 1860 No. 122 of 1966-1967, Lucknow)

Indian Journal of Pharmaceutical Education and Research (IJPER) [ISSN-0019-5464] is the official journal of Association of Pharmaceutical Teachers of India (APTI) and is being published since 1967.

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